Cryptosporidium species detected in calves and cattle in Dagoretti, Nairobi, Kenya

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dc.contributor.authorKang’ethe, Erastus K.
dc.contributor.authorMulinge, Erastus K.
dc.contributor.authorSkilton, Robert A.
dc.contributor.authorNjahira, Moses
dc.contributor.authorMonda, Joseph G.
dc.date.accessioned2012-09-07T21:31:41Z
dc.date.available2012-09-07T21:31:41Z
dc.date.copyright2012
dc.date.issued2012-07
dc.descriptionThis paper is part of a special supplement on assessing and managing urban zoonoses and food-borne disease in two African cities (Nairobi, Kenya and Ibadan, Nigeria).en
dc.description.abstractA total of 1,734 cattle faecal samples from 296 dairy-keeping households were collected from urban settings in Nairobi, Kenya. Modified Ziehl–Neelsen staining method and an immunofluorescence assay were used to identify those samples with Cryptosporidium oocyst infection. Oocysts from positive faecal samples were isolated by Sheather's sucrose flotation method and picked from the concentrate using cover slips. Genomic DNA was extracted from 124 of the faecal samples that were positive for Cryptosporidium and was used as template for nested PCR of the 18S rRNA gene. Twentyfive samples (20 %) were PCR-positive for Cryptosporidium, and 24 of the PCR products were successfully cloned and sequenced. Sequence and phylogenetic analysis identified 17 samples (68 %) as Cryptosporidium parvum-like, four samples (16 %) as Cryptosporidium ryanae, three samples (12 %) as Cryptosporidium andersoni and one sample (4 %) as Cryptosporidium hominis. To the best of our knowledge, this is the first genotyping study to report C. parvum-like, C. andersoni and C. hominis in cattle from Kenya. The results of this study show Cryptosporidium infections in calves and cattle may be potential zoonotic reservoirs of the parasite that infects humans.en
dc.formatTexten
dc.format.extent1 digital file (9 p. : ill.)en
dc.format.mimetypeapplication/pdf
dc.identifier.citationKang’ethe, E.K., Mulinge, E.K., Skilton, R.A., Njahira, M., Monda, J.G., et al. (2012). Cryptosporidium species detected in calves and cattle in Dagoretti, Nairobi, Kenya. Tropical Animal Health and Production, SI.doi:10.1007/s11250-012-0202-5en
dc.identifier.issn0049-4747
dc.identifier.urihttp://hdl.handle.net/10625/50250
dc.language.isoen
dc.publisherSpringeren
dc.relation.journalTropical Animal Health and Production
dc.subjectCRYPTOSPORIDIUMen
dc.subjectGENOTYPINGen
dc.subjectDAIRY FARMINGen
dc.subjectKENYAen
dc.subjectURBAN DAIRY PRODUCTIONen
dc.subjectZOONOTIC DISEASESen
dc.subjectFOOD BORNE DISEASEen
dc.subjectLIVESTOCKen
dc.titleCryptosporidium species detected in calves and cattle in Dagoretti, Nairobi, Kenyaen
dc.typeJournal Article (peer-reviewed)en
idrc.copyright.holderSpringer Science+Business Media B.V.
idrc.dspace.accessIDRC Onlyen
idrc.noaccessDue to copyright restrictions the full text of this research output is not available in the IDRC Digital Library or by request from the IDRC Library. / Compte tenu des restrictions relatives au droit d'auteur, le texte intégral de cet extrant de recherche n'est pas accessible dans la Bibliothèque numérique du CRDI, et il n'est pas possible d'en faire la demande à la Bibliothéque du CRDI.en
idrc.project.componentnumber103075001
idrc.project.number103075
idrc.project.titleHealth Risk Analysis of Cryptosporidiosis and other Hazards in Urban Smallholder Dairy Production (Kenya)en
idrc.rims.adhocgroupIDRC SUPPORTEDen

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