Quantitative resistance in potato leaves to late blight associated with induced hydroxycinnamic acid amides

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dc.contributor.authorYogendra, Kalenahalli N.
dc.contributor.authorPushpa, Doddaraju
dc.contributor.authorMosa, Kareem A.
dc.contributor.authorKushalappa, Ajjamada C.
dc.contributor.authorMurphy, Agnes
dc.contributor.authorMosquera, Teresa
dc.date.accessioned2015-02-09T19:49:11Z
dc.date.available2015-02-09T19:49:11Z
dc.date.copyright2014
dc.date.issued2014-01
dc.description.abstractLate blight is a serious economic threat to potato crop, sometimes leading to complete crop loss. The resistance in potato to late blight can be qualitative or quantitative in nature. Qualitative resistance is not durable. Though quantitative resistance is durable, the breeding is challenging due to polygenic inheritance. Several quantitative trait loci (QTLs) have been identified, but the mechanisms of resistance are largely unknown. A nontargeted metabolomics approach was used to identify resistance-related (RR) metabolites in a resistant genotype (F06025), as compared to a susceptible (Shepody) genotype, mock- or pathogen-inoculated. The RR metabolites, which had high fold change in abundance, mainly belonged to phenylpropanoid, flavonoid, fatty acid, and alkaloid chemical groups. The most important phenylpropanoids identified were hydroxycinnamic acid amides, the polyaromatic domain of suberin that is known to be associated with cell wall reinforcement. These metabolites were mapped on to the potato metabolic pathways, and the candidate enzymes and their coding genes were identified. A quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay revealed a higher upregulation of 4-coumarate: CoA ligase (4-CL), tyrosine decarboxylase (TyDC), and tyramine hydroxycinnamoyl transferase (THT) in the pathogen-inoculated resistant genotype than in susceptible. These genes were sequenced in both resistant and susceptible genotypes, and nonsynonymous single-nucleotide polymorphisms (nsSNPs) were found. The application of these genes in potato resistance improvement, following validation, is discussed.en
dc.formatTexten
dc.format.extent1 digital file (p. 285-298)en
dc.format.mimetypeApplication/pdf
dc.identifier.citationYogendra, K.N., Pushpa, D., Mosa, K.A., Kushalappa, A.C., Murphy, A., & Mosquera, T. (2014). Quantitative resistance in potato leaves to late blight associated with induced hydroxycinnamic acid amides. Functional & Integrative Genomics, 14(2): 285-298. doi:10.1007/s10142-013-0358-8en
dc.identifier.urihttp://hdl.handle.net/10625/53666
dc.language.isoen
dc.publisherSpringeren
dc.subjectMETABOLOMICSen
dc.subjectQUANTITATIVE RESISTANCEen
dc.subjectPOTATO LATE BLIGHTen
dc.subjectPHYTOPHTHORA INFESTANSen
dc.subjectPOLYGENIC RESISTANCEen
dc.subjectSINGLE NUCLEOTIDE POLYMORPHISMen
dc.subjectCROP DAMAGEen
dc.subjectAGRICULTUREen
dc.subjectPOTATOESen
dc.subjectPLANT GENETICSen
dc.titleQuantitative resistance in potato leaves to late blight associated with induced hydroxycinnamic acid amidesen
dc.typeJournal Article (peer-reviewed)en
idrc.copyright.holderSpringer-Verlag Berlin Heidelberg
idrc.dspace.accessIDRC Onlyen
idrc.noaccessDue to copyright restrictions the full text of this research output is not available in the IDRC Digital Library or by request from the IDRC Library. / Compte tenu des restrictions relatives au droit d'auteur, le texte intégral de cet extrant de recherche n'est pas accessible dans la Bibliothèque numérique du CRDI, et il n'est pas possible d'en faire la demande à la Bibliothéque du CRDI.en
idrc.project.number106926
idrc.project.titleImproving Potato Production for Increased Food Security of Indigenous Communities in Colombia (CIFSRF)en
idrc.recordsserver.bcsnumberIC01-4418-156
idrc.rims.adhocgroupIDRC SUPPORTEDen

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