Waterborne copper exposure inhibits ammonia excretion and branchial carbonic anhydrase activity in euryhaline guppies acclimated to both fresh water and sea water

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dc.contributor.authorZimmer, Alex
dc.contributor.authorIndianara Fernanda Barcarolli
dc.contributor.authorChris M. Wood
dc.contributor.authorAdalto Bianchini
dc.date.accessioned2013-08-09T12:47:29Z
dc.date.available2013-08-09T12:47:29Z
dc.date.copyright2012
dc.date.issued2012
dc.description.abstractInhibition of ammonia excretion (J(amm)) is a common response to Cu exposure in freshwater (FW) and seawater (SW) organisms. To determine the mechanism of this response, a euryhaline species of guppy (Poecilia vivipara) was exposed to 20 μg Cu/l in FW (0 ppt) and SW (25 ppt) for 96 h. In both salinities, Cu transiently inhibited ammonia excretion (J(amm)) followed by a full recovery by the end of the 96 h exposure. The activities of Na(+)/K(+)-ATPase, H(+)-ATPase, and carbonic anhydrase (CA) were examined in the gills at 12 and 96 h of Cu exposure. In both salinity acclimations, CA activity was significantly inhibited following 12h of Cu exposure in P. vivipara, marking the first in vivo evidence of Cu-induced inhibition of CA in fish. Moreover, the inhibition and recovery of this enzyme were correlated with the inhibition and recovery of J(amm) in both salinity acclimations. The blockade of CA potentially acts as a common mechanism of J(amm) inhibition in FW and SW. There were no significant effects on Na(+)/K(+)-ATPase or H(+)-ATPase activity at either time point or salinity. However, H(+)-ATPase activity was upregulated at 96 h relative to the 12h time point, potentially involving this enzyme in re-establishing J(amm).en
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dc.identifier.citationZimmer, A.M., Barcarolli, I.F., Wood, C.M., & Bianchini, A. (2012). Waterborne copper exposure inhibits ammonia excretion and branchial carbonic anhydrase activity in euryhaline guppies acclimated to both fresh water and sea water. Aquatic Toxicology, 122-123, 172-180.doi:10.1016/j.aquatox.2012.06.010en
dc.identifier.issn0166-445X
dc.identifier.urihttp://hdl.handle.net/10625/51487
dc.language.isoen
dc.publisherElsevieren
dc.subjectCOPPERen
dc.subjectCARBONIC ANHYDRASEen
dc.subjectAMMONIA EXCRETION (JAMM)en
dc.subjectEURYHALINEen
dc.subjectPOECILIA VIVIPARAen
dc.subjectFISHen
dc.subjectSODIUM-POTASSIUM EXCHANGING ATPASEen
dc.titleWaterborne copper exposure inhibits ammonia excretion and branchial carbonic anhydrase activity in euryhaline guppies acclimated to both fresh water and sea wateren
dc.typeJournal Article (peer-reviewed)en
idrc.copyright.holderElsevier B.V.
idrc.dspace.accessIDRC Onlyen
idrc.noaccessDue to copyright restrictions the full text of this research output is not available in the IDRC Digital Library or by request from the IDRC Library. / Compte tenu des restrictions relatives au droit d'auteur, le texte intégral de cet extrant de recherche n'est pas accessible dans la Bibliothèque numérique du CRDI, et il n'est pas possible d'en faire la demande à la Bibliothéque du CRDI.en
idrc.project.componentnumber104519011
idrc.project.number104519
idrc.project.titleInternational Research Chairs Initiative (IRCI)en
idrc.recordsserver.bcsnumberIC01-3412-154
idrc.rims.adhocgroupIDRC SUPPORTEDen

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