Method-comparison study of a hemoglobinometer and a hematology analyzer to measure hemoglobin concentration among women with genetic hemoglobin disorders in Cambodia

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dc.contributor.authorKarakochuk, Crystal D.
dc.contributor.authorJanmohamed, Amynah
dc.contributor.authorWhitfield, Kyly C.
dc.contributor.authorBarr, Susan I.
dc.contributor.authorVercauteren, Suzanne M.
dc.date.accessioned2015-02-25T20:11:42Z
dc.date.available2015-02-25T20:11:42Z
dc.date.issued2014-09
dc.description.abstractBackground: Genetic hemoglobin (Hb) E variants are common in Cambodia and result in an altered and unstable Hb molecule. There are no known studies on the accuracy of Hb measurement among individuals with Hb E or other Hb variants. Methods: This method-comparison study evaluates two methods to measure Hb concentration in capillary blood using a hemoglobinometer (HemoCueÒ) and in venous blood using an automated hematology analyzer (Sysmex XT-1800i). We determined the bias and concordance between the two methods among 420 non-pregnant women (18-45 y) in Cambodia using secondary data from a separate trial. Results: Bias and concordance appeared similar between methods among women with no Hb disorders (n=195, bias=2.5, rc=0.68), women with Hb E variants (n=133, bias=2.5, rc=0.78), and women with other Hb variants (n=92, bias=2.7, rc=0.73). Overall, agreement was poor between methods. The overall bias was 2.6 g/L (difference in Hb means between methods), resulting in a difference in anemia prevalence of 11.5% (41% using HemoCueÒ and 29.5% using Sysmex, p<0.001). This changes the WHO anemia severity from a ‘moderate’ to a ‘severe’ public health problem. Based on concordance plots, the HemoCueÒ device appears to underestimate Hb concentrations in capillary blood as compared to Sysmex (venous blood) at lower Hb concentrations, and to overestimate Hb concentrations in capillary blood as compared to Sysmex at higher Hb concentrations. Conclusions: Bias and concordance were similar among all groups, suggesting the two methods of Hb measurement were comparable. We highlight the bias between the two methods to caution field staff, researchers and policy makers in the interpretation of data and the impact that even a small bias between methods can have on anemia prevalence rates.en
dc.formatTexten
dc.format.extent1 digital file (30 p. : ill.)en
dc.format.mimetypeApplication/pdf
dc.identifier.urihttp://hdl.handle.net/10625/53778
dc.language.isoen
dc.subjectANEMIAen
dc.subjectHEMOCUEen
dc.subjectHEMOGLOBINOMETERen
dc.subjectHEMOGLOBIN DISORDERSen
dc.subjectBIASen
dc.subjectCAMBODIAen
dc.subject50 METHOD-COMPARISONen
dc.subjectHEMOGLOBINen
dc.subjectWOMEN'S HEALTHen
dc.subjectHEALTH SURVEYSen
dc.titleMethod-comparison study of a hemoglobinometer and a hematology analyzer to measure hemoglobin concentration among women with genetic hemoglobin disorders in Cambodiaen
dc.title.alternativeKarakochuk - BMC Public Health manuscript - Sept 2014 (annex 18a)en
dc.typeJournal Article (peer-reviewed)en
idrc.dspace.accessIDRC Onlyen
idrc.noaccessDue to copyright restrictions the full text of this research output is not available in the IDRC Digital Library or by request from the IDRC Library. / Compte tenu des restrictions relatives au droit d'auteur, le texte intégral de cet extrant de recherche n'est pas accessible dans la Bibliothèque numérique du CRDI, et il n'est pas possible d'en faire la demande à la Bibliothéque du CRDI.en
idrc.project.componentnumber106928001
idrc.project.number106928
idrc.project.titleSmall-scale Aquaculture to Strengthen Food Security in Cambodia (CIFSRF)en
idrc.recordsserver.bcsnumberIC01-971-165
idrc.rims.adhocgroupIDRC SUPPORTEDen

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