Qing-long LiangKai ZhouHong-xuan He2012-08-292012-08-292010Qing-long Liang, Kai Zhou, & Hong-xuan He (2010). Retrocyclin 2: a new therapy against avian influenza H5N1 virus in vivo and vitro. Biotechnol Lett, 32, 387-392. doi:10.1007/s10529-009-0167-2http://hdl.handle.net/10625/50228We tested the ability of retrocyclin 2, a type of h defensin, to protect cells and chicken embryos from infection by H5N1 highly pathogenic avian influenza virus. A gene fragment of retrocyclin 2 was designed based on the protein sequence of retrocyclin 2 and cloned into the eukaryotic expression vector pcDNA4.01 (HismaxA), named pcDNA4-RC2. The expression vector pcDNA4-RC2 protected MDCK cells and chicken embryos from infection by the H5N1 virus through inhibition of virus replication and viral mRNA transcription. Retrocyclin 2 is therefore effective in preventing H5N1 virus infection in vivo and in vitro and could be considered as a new therapy for H5N1 influenza and other diseases.Text1 digital file (p. 387-392)Application/pdfenAVIAN INFLUENZA VIRUSCHICKEN EMBRYOSH5N1INFLUENZA VIRUSMDCK CELLSRETROCYCLIN 2INFLUENZA IN BIRDSINFLUENZA A VIRUS, H5N1 SUBTYPEJournal Article (peer-reviewed)