Assessment of EST-microsatellites markers for discrimination and genetic diversity in bread and durum wheat landraces from Afghanistan

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dc.contributor.authorChabane, K.
dc.contributor.authorAbdalla, O.
dc.contributor.authorSayed, H.
dc.contributor.authorValkoun, J.
dc.date.accessioned2007-11-13T08:29:45Z
dc.date.available2007-10-17en
dc.date.available2007-11-13T08:29:45Z
dc.date.issued2007
dc.description.abstractAbstract Accurate and reliable means for identification are necessary to assess the discrimination between landraces of tetraploid wheat [T. turgidum L. subsp. durum (Desf.) Husn.] and hexaploid wheat (T. aestivum L. em. Thell.). In Afghanistan, farmers usually cultivate mixed landraces, and thus distinction between bread and durum is difficult. A set of 18 microsatellites derived from the DuPont ESTdatabase were used to describe genetic diversity in a sample of 82 Afghan wheat landraces. A total of 101 alleles were detected, with allele number per locus ranging from 2 to 13, and a mean allele number of 6.31. The percentage of polymorphic loci was 89%. The EST-SSRs markers showed different level of gene diversity: the highest Polymorphism Information Content value (0.921) was observed with DuPw 221. Our results demonstrated that with a reasonable number of expressed sequences target microsatellites (EST-SSRs) it is possible to discriminate between T. durum and T. aestivum species of wheat germplasm. Our results showed that ESTdatabases could be a useful source for speciesspecific markers and have the potential for new genic microsatellites markers that could enhance screening germplasm in gene banks.en
dc.formatTexten
dc.format.extent1 digital file (8 p. : ill.)en
dc.identifier.urihttp://hdl.handle.net/10625/34506
dc.language.isoen
dc.publisherSpringer, Dordrecht, NLen
dc.relation.ispartofGenetic resources and crop evolutionen
dc.subjectMICROSATELLITESen
dc.subjectEST-SSRen
dc.subjectSPECIES DISCRIMINATIONen
dc.subjectLANDRACESen
dc.subjectREMOTE SENSINGen
dc.subjectWHEATen
dc.subjectBIODIVERSITYen
dc.subjectAFGHANISTANen
dc.titleAssessment of EST-microsatellites markers for discrimination and genetic diversity in bread and durum wheat landraces from Afghanistanen
dc.typeJournal Article (peer-reviewed)en
idrc.dspace.accessIDRC Onlyen
idrc.project.number101344
idrc.project.titleStrengthening Seed Systems for Food Security in Afghanistanen
idrc.rims.adhocgroupIDRC SUPPORTEDen

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