Method-comparison study of a hemoglobinometer and a hematology analyzer to measure hemoglobin concentration among women with genetic hemoglobin disorders in Cambodia

Show simple item record

dc.contributor.author Karakochuk, Crystal D.
dc.contributor.author Janmohamed, Amynah
dc.contributor.author Whitfield, Kyly C.
dc.contributor.author Barr, Susan I.
dc.contributor.author Vercauteren, Suzanne M.
dc.date.accessioned 2015-02-25T20:11:42Z
dc.date.available 2015-02-25T20:11:42Z
dc.date.issued 2014-09
dc.identifier.uri http://hdl.handle.net/10625/53778
dc.description.abstract Background: Genetic hemoglobin (Hb) E variants are common in Cambodia and result in an altered and unstable Hb molecule. There are no known studies on the accuracy of Hb measurement among individuals with Hb E or other Hb variants. Methods: This method-comparison study evaluates two methods to measure Hb concentration in capillary blood using a hemoglobinometer (HemoCueÒ) and in venous blood using an automated hematology analyzer (Sysmex XT-1800i). We determined the bias and concordance between the two methods among 420 non-pregnant women (18-45 y) in Cambodia using secondary data from a separate trial. Results: Bias and concordance appeared similar between methods among women with no Hb disorders (n=195, bias=2.5, rc=0.68), women with Hb E variants (n=133, bias=2.5, rc=0.78), and women with other Hb variants (n=92, bias=2.7, rc=0.73). Overall, agreement was poor between methods. The overall bias was 2.6 g/L (difference in Hb means between methods), resulting in a difference in anemia prevalence of 11.5% (41% using HemoCueÒ and 29.5% using Sysmex, p<0.001). This changes the WHO anemia severity from a ‘moderate’ to a ‘severe’ public health problem. Based on concordance plots, the HemoCueÒ device appears to underestimate Hb concentrations in capillary blood as compared to Sysmex (venous blood) at lower Hb concentrations, and to overestimate Hb concentrations in capillary blood as compared to Sysmex at higher Hb concentrations. Conclusions: Bias and concordance were similar among all groups, suggesting the two methods of Hb measurement were comparable. We highlight the bias between the two methods to caution field staff, researchers and policy makers in the interpretation of data and the impact that even a small bias between methods can have on anemia prevalence rates. en
dc.format Text en
dc.format.extent 1 digital file (30 p. : ill.) en
dc.format.mimetype Application/pdf
dc.language.iso en
dc.subject ANEMIA en
dc.subject HEMOCUE en
dc.subject HEMOGLOBINOMETER en
dc.subject HEMOGLOBIN DISORDERS en
dc.subject BIAS en
dc.subject CAMBODIA en
dc.subject 50 METHOD-COMPARISON en
dc.subject HEMOGLOBIN en
dc.subject WOMEN'S HEALTH en
dc.subject HEALTH SURVEYS en
dc.title Method-comparison study of a hemoglobinometer and a hematology analyzer to measure hemoglobin concentration among women with genetic hemoglobin disorders in Cambodia en
dc.title.alternative Karakochuk - BMC Public Health manuscript - Sept 2014 (annex 18a) en
dc.type Journal Article (peer-reviewed) en
idrc.project.number 106928
idrc.project.componentnumber 106928001
idrc.project.title Small-scale Aquaculture to Strengthen Food Security in Cambodia (CIFSRF) en
idrc.dspace.access IDRC Only en
idrc.rims.adhocgroup IDRC SUPPORTED en
idrc.recordsserver.bcsnumber IC01-971-165
idrc.noaccess Due to copyright restrictions the full text of this research output is not available in the IDRC Digital Library or by request from the IDRC Library. / Compte tenu des restrictions relatives au droit d'auteur, le texte intégral de cet extrant de recherche n'est pas accessible dans la Bibliothèque numérique du CRDI, et il n'est pas possible d'en faire la demande à la Bibliothéque du CRDI. en


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record