Quantitative resistance in potato leaves to late blight associated with induced hydroxycinnamic acid amides

Date

2014-01

Authors

Journal Title

Journal ISSN

Volume Title

Publisher

Springer

Abstract

Late blight is a serious economic threat to potato crop, sometimes leading to complete crop loss. The resistance in potato to late blight can be qualitative or quantitative in nature. Qualitative resistance is not durable. Though quantitative resistance is durable, the breeding is challenging due to polygenic inheritance. Several quantitative trait loci (QTLs) have been identified, but the mechanisms of resistance are largely unknown. A nontargeted metabolomics approach was used to identify resistance-related (RR) metabolites in a resistant genotype (F06025), as compared to a susceptible (Shepody) genotype, mock- or pathogen-inoculated. The RR metabolites, which had high fold change in abundance, mainly belonged to phenylpropanoid, flavonoid, fatty acid, and alkaloid chemical groups. The most important phenylpropanoids identified were hydroxycinnamic acid amides, the polyaromatic domain of suberin that is known to be associated with cell wall reinforcement. These metabolites were mapped on to the potato metabolic pathways, and the candidate enzymes and their coding genes were identified. A quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay revealed a higher upregulation of 4-coumarate: CoA ligase (4-CL), tyrosine decarboxylase (TyDC), and tyramine hydroxycinnamoyl transferase (THT) in the pathogen-inoculated resistant genotype than in susceptible. These genes were sequenced in both resistant and susceptible genotypes, and nonsynonymous single-nucleotide polymorphisms (nsSNPs) were found. The application of these genes in potato resistance improvement, following validation, is discussed.

Description

Keywords

METABOLOMICS, QUANTITATIVE RESISTANCE, POTATO LATE BLIGHT, PHYTOPHTHORA INFESTANS, POLYGENIC RESISTANCE, SINGLE NUCLEOTIDE POLYMORPHISM, CROP DAMAGE, AGRICULTURE, POTATOES, PLANT GENETICS

Citation

Yogendra, K.N., Pushpa, D., Mosa, K.A., Kushalappa, A.C., Murphy, A., & Mosquera, T. (2014). Quantitative resistance in potato leaves to late blight associated with induced hydroxycinnamic acid amides. Functional & Integrative Genomics, 14(2): 285-298. doi:10.1007/s10142-013-0358-8

URI

http://hdl.handle.net/10625/53666

DOI

Collections

IDRC Research Results / Résultats de recherches du CRDI
2010-2019 / Années 2010-2019
Research Results (AFS) / Résultats de recherche (ASA)
Research Results (CIFSRF) / Résultats de recherches (FCRSAI)