Cryptosporidium species detected in calves and cattle in Dagoretti, Nairobi, Kenya

Show simple item record

dc.contributor.author Kang’ethe, Erastus K.
dc.contributor.author Mulinge, Erastus K.
dc.contributor.author Skilton, Robert A.
dc.contributor.author Njahira, Moses
dc.contributor.author Monda, Joseph G.
dc.date.accessioned 2012-09-07T21:31:41Z
dc.date.available 2012-09-07T21:31:41Z
dc.date.copyright 2012
dc.date.issued 2012-07
dc.identifier.citation Kang’ethe, E.K., Mulinge, E.K., Skilton, R.A., Njahira, M., Monda, J.G., et al. (2012). Cryptosporidium species detected in calves and cattle in Dagoretti, Nairobi, Kenya. Tropical Animal Health and Production, SI.doi:10.1007/s11250-012-0202-5 en
dc.identifier.issn 0049-4747
dc.identifier.uri http://hdl.handle.net/10625/50250
dc.description This paper is part of a special supplement on assessing and managing urban zoonoses and food-borne disease in two African cities (Nairobi, Kenya and Ibadan, Nigeria). en
dc.description.abstract A total of 1,734 cattle faecal samples from 296 dairy-keeping households were collected from urban settings in Nairobi, Kenya. Modified Ziehl–Neelsen staining method and an immunofluorescence assay were used to identify those samples with Cryptosporidium oocyst infection. Oocysts from positive faecal samples were isolated by Sheather's sucrose flotation method and picked from the concentrate using cover slips. Genomic DNA was extracted from 124 of the faecal samples that were positive for Cryptosporidium and was used as template for nested PCR of the 18S rRNA gene. Twentyfive samples (20 %) were PCR-positive for Cryptosporidium, and 24 of the PCR products were successfully cloned and sequenced. Sequence and phylogenetic analysis identified 17 samples (68 %) as Cryptosporidium parvum-like, four samples (16 %) as Cryptosporidium ryanae, three samples (12 %) as Cryptosporidium andersoni and one sample (4 %) as Cryptosporidium hominis. To the best of our knowledge, this is the first genotyping study to report C. parvum-like, C. andersoni and C. hominis in cattle from Kenya. The results of this study show Cryptosporidium infections in calves and cattle may be potential zoonotic reservoirs of the parasite that infects humans. en
dc.format Text en
dc.format.extent 1 digital file (9 p. : ill.) en
dc.format.mimetype application/pdf
dc.language.iso en
dc.publisher Springer en
dc.subject CRYPTOSPORIDIUM en
dc.subject GENOTYPING en
dc.subject DAIRY FARMING en
dc.subject KENYA en
dc.subject URBAN DAIRY PRODUCTION en
dc.subject ZOONOTIC DISEASES en
dc.subject FOOD BORNE DISEASE en
dc.subject LIVESTOCK en
dc.title Cryptosporidium species detected in calves and cattle in Dagoretti, Nairobi, Kenya en
dc.type Journal Article (peer-reviewed) en
idrc.project.number 103075
idrc.project.componentnumber 103075001
idrc.project.title Health Risk Analysis of Cryptosporidiosis and other Hazards in Urban Smallholder Dairy Production (Kenya) en
idrc.copyright.holder Springer Science+Business Media B.V.
idrc.dspace.access IDRC Only en
idrc.rims.adhocgroup IDRC SUPPORTED en
dc.relation.journal Tropical Animal Health and Production
idrc.noaccess Due to copyright restrictions the full text of this research output is not available in the IDRC Digital Library or by request from the IDRC Library. / Compte tenu des restrictions relatives au droit d'auteur, le texte intégral de cet extrant de recherche n'est pas accessible dans la Bibliothèque numérique du CRDI, et il n'est pas possible d'en faire la demande à la Bibliothéque du CRDI. en


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record