Retrocyclin 2 : a new therapy against avian influenza H5N1 virus in vivo and vitro

Date

2010

Journal Title

Journal ISSN

Volume Title

Publisher

Springer

Abstract

We tested the ability of retrocyclin 2, a type of h defensin, to protect cells and chicken embryos from infection by H5N1 highly pathogenic avian influenza virus. A gene fragment of retrocyclin 2 was designed based on the protein sequence of retrocyclin 2 and cloned into the eukaryotic expression vector pcDNA4.01 (HismaxA), named pcDNA4-RC2. The expression vector pcDNA4-RC2 protected MDCK cells and chicken embryos from infection by the H5N1 virus through inhibition of virus replication and viral mRNA transcription. Retrocyclin 2 is therefore effective in preventing H5N1 virus infection in vivo and in vitro and could be considered as a new therapy for H5N1 influenza and other diseases.

Description

Keywords

AVIAN INFLUENZA VIRUS, CHICKEN EMBRYOS, H5N1, INFLUENZA VIRUS, MDCK CELLS, RETROCYCLIN 2, INFLUENZA IN BIRDS, INFLUENZA A VIRUS, H5N1 SUBTYPE

Citation

Qing-long Liang, Kai Zhou, & Hong-xuan He (2010). Retrocyclin 2: a new therapy against avian influenza H5N1 virus in vivo and vitro. Biotechnol Lett, 32, 387-392. doi:10.1007/s10529-009-0167-2

DOI