Assessment of EST-microsatellites markers for discrimination and genetic diversity in bread and durum wheat landraces from Afghanistan
Date
2007
Authors
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Publisher
Springer, Dordrecht, NL
Abstract
Abstract Accurate and reliable means for
identification are necessary to assess the discrimination
between landraces of tetraploid
wheat [T. turgidum L. subsp. durum (Desf.)
Husn.] and hexaploid wheat (T. aestivum L. em.
Thell.). In Afghanistan, farmers usually cultivate
mixed landraces, and thus distinction between
bread and durum is difficult. A set of 18 microsatellites
derived from the DuPont ESTdatabase
were used to describe genetic diversity
in a sample of 82 Afghan wheat landraces. A
total of 101 alleles were detected, with allele
number per locus ranging from 2 to 13, and a
mean allele number of 6.31. The percentage of
polymorphic loci was 89%. The EST-SSRs
markers showed different level of gene diversity:
the highest Polymorphism Information Content
value (0.921) was observed with DuPw 221. Our
results demonstrated that with a reasonable
number of expressed sequences target microsatellites
(EST-SSRs) it is possible to discriminate
between T. durum and T. aestivum species of
wheat germplasm. Our results showed that ESTdatabases
could be a useful source for speciesspecific
markers and have the potential for new
genic microsatellites markers that could enhance
screening germplasm in gene banks.
Description
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Journal Article (peer-reviewed)
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Keywords
MICROSATELLITES, EST-SSR, SPECIES DISCRIMINATION, LANDRACES, REMOTE SENSING, WHEAT, BIODIVERSITY, AFGHANISTAN